Eppendorf BioFlo 120 Advanced Bioreactor Fermenter System w/ 5L Vessel

Eppendorf BioFlo 120 Advanced Bioreactor Fermenter System w/ 5L Vessel

Model: BioFlo 120, 0-20SLPM, 1x TMFC
MFG: 2022
Power: 100-240VAC, 50/60Hz
Vessel: 5L glass autoclavable single-walled, working volume: 2.0 - 5.6L
Application: Microbio

Condition: Used, tested in good working condition. System was throughly tested for all functions, including: pumps, gassing, agitation, temp, measurement, & water circulation. Vessel was disassembled, sanitized, & cleaned.  Headplate and headplate adapter o-rings and ferrules were replaced.  Shaft bearings, o-rings, & ferrules were replaced.  Glass is in good condition, free of chips. All sensors were tested and calibrated giving results within the expected range.

Includes:

• Bioflo 120 Advanced Controller (1390100000)
  • 0-20 slpm
  • 1x TMFC
  • Analog I/O
• 5L Single Wall Glass Vessel, direct drive (1390 400 200)
  • (2x) Rushton Impellers (M1273-9292)
  • Cooling Ring (M1273-9247)
  • Baffle (M1273-9245)
  • Thermowell (M1273-9202)
  • Harvest Tube (M1273-9162)
  • Sample Tube (M1273-9170)
  • Ring Sparger, Macro (M1273-9246)
  • Exhaust Condenser (1390502000)
  • Heat Blanket (13908720200)
• Vessel Headplate Adapters
  • (2x) compression fitting, 12mm port to 12mm sensor (M1273-5040)
    
  • (2x) compression fitting for sample tube/ level sensor (M1273-5042)
    
  • (4x) 12mm port to 6mm tube (M1273-5056)
    • (2x) for cooling ring
    • (1x) for sparger
    • (1x) for harvest tube
  • 12mm port to 12mm tube for exhaust condenser (M1273-5058)
    
  • (1x) 6mm Blind Plugs (M1273-9405)
  • (2x) 12mm Blind Plugs (M1273-9406)
  • 19mm Blind Plug (M1273-9407)
  • Triple Port (M1273-9961)
  • Syringe Port adapter w/ septum (M1273-3031)
• Sensors
  
  • Mettler Analog pH Sensor 405-DPAS-SC-K83/325
    • Analog pH Cable K9 (1390810400)
  • Mettler Optical DO Sensor 6860i/12/320/nA
    • Optical DO Cable VP8, ISM (M1379-8107)
  • Level Sensor (1390500200)
    • Level Sensor Cable (1390611000)
  • Temp Sensor (1390810000)
• Additional Accessories
  • Direct Drive Agitation Motor (1390080000)
    
  • 4-Gas Regulator (M1363-5002)
  • 120v Power Cord (P0720-2024)
  • Ground Cable (1390811200)
  • Water connection kit
    • Tubing from chiller (chiller not included) to controller
      • chiller side open connection to add your connector
    • Tubing from controller to exhaust condenser
    • Tubing from controller to cooling ring
  • Gas connection kit
    • Tubing from the regulator to the controller inputs
    • Tubing from the controller sparge output to sparger
  • Operations Manual on USB Drive

Description:

The Eppendorf BioFlo 120 is a bench-scale bioprocess control station designed for research and process development in both microbial fermentation and cell culture workflows. Eppendorf positions the platform as a flexible R&D system that can be used with reusable glass vessels as well as BioBLU single-use bioreactors, making it a strong fit for labs that want a controller that can adapt to different applications over time.

This configuration is especially well suited for fermentation labs that want a compact, capable system for bench-scale cultivation, optimization, and process development. Your listed unit includes a glass vessel setup for microbial work along with the accessories needed for agitation, gassing, temperature control, and measurement, making it a practical choice for users who want a complete working platform rather than a bare controller. The BioFlo 120 is also designed to support batch, fed-batch, and continuous or perfusion-style processes, which adds useful flexibility for development work.

A major strength of the BioFlo 120 platform is versatility. Eppendorf highlights its compatibility with a wide variety of autoclavable and single-use bioreactors, along with support for both sensitive mammalian cell culture and more demanding microbial cultures on the same controller. For buyers who want a system that can support evolving workflows without stepping into a much larger pilot platform, that flexibility is one of the BioFlo 120’s biggest advantages.

Its compact footprint and straightforward setup also make it appealing for academic, government, and industrial labs that need strong process control without giving up valuable bench space. In practical terms, it is a good fit for organizations looking for an established Eppendorf bioprocess platform for fermentation development, small-scale cultivation, and day-to-day R&D use.

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